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MYH9 Monoclonal antibody
MYH9 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig
Applications
WB, IF/ICC, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
5D9D2
验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human kidney tissue, pig spleen tissue, U2OS cells, mouse colon tissue, pig pancreas tissue, A431 cells, human placenta tissue, pig kidney tissue |
| Positive IF/ICC detected in | HepG2 cells, U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60233-1-Ig targets MYH9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse |
| Immunogen | MYH9 fusion protein Ag1612 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | myosin, heavy chain 9, non-muscle |
| Synonyms | DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9 |
| Calculated Molecular Weight | 227 kDa |
| Observed Molecular Weight | 230 kDa, 160 kDa |
| GenBank Accession Number | BC011915 |
| Gene Symbol | MYH9 |
| Gene ID (NCBI) | 4627 |
| RRID | AB_2881357 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35579 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH9 antibody 60233-1-Ig | Download protocol |
| IF protocol for MYH9 antibody 60233-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. | ||
Cell Death Differ Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling
| ||
Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells | ||
Theranostics Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. | ||
J Autoimmun Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu. |
