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  • KD/KO Validated

MYH9 Monoclonal antibody

MYH9 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

5D9D2

Cat no : 60233-1-Ig

Print datasheet

Synonyms

DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9



经过测试的应用

Positive WB detected inmouse kidney tissue, human kidney tissue, pig spleen tissue, U2OS cells, mouse colon tissue, pig pancreas tissue, A431 cells, human placenta tissue, pig kidney tissue
Positive IF/ICC detected inHepG2 cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60233-1-Ig targets MYH9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse
Immunogen MYH9 fusion protein Ag1612 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 9, non-muscle
Synonyms DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9
Calculated Molecular Weight 227 kDa
Observed Molecular Weight 230 kDa, 160 kDa
GenBank Accession NumberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
RRIDAB_2881357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

实验方案

Product Specific Protocols
WB protocol for MYH9 antibody 60233-1-IgDownload protocol
IF protocol for MYH9 antibody 60233-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.

Authors - Pasquale Cervero
humanWB,IHC,IF

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
  • KD Validated
humanWB,CoIP,IF

Cell Death Differ

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling

Authors - Shunfeng Hu
  • KD Validated
human,mouseWB,IHC,IF

Cell Rep

Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells

Authors - Yingqi Liu
mouseIF

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
humanIF

J Autoimmun

Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu.

Authors - Dongdong Zhang