Mouse CXCL2 ELISA Kit

Catalog number: KE10022

规格

单价

96 T

¥2600

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Overview


Product name:
Mouse CXCL2 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
20.45 pg/mL

Range:
31.25 - 500 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 86% 72%-133%
Cell culture supernatant 100% 82%-121%

Product overview:
KE10022 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL2 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL2. The assay recognizes mouse CXCL2. A monoclonal antibody specific for CXCL2 has been pre-coated onto the microwells. The CXCL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for CXCL2 is added to detect the captured CXCL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store the standard and plate at -20˚. The rest of the kit should be stored at 4˚. Refer to the protocol for further storage instructions

Synonyms:
CINC-2a; GRO2; GROb; MGSA-b; MIP-2a; MIP2; MIP2A; SCYB2
Background

Cxcl2, also known as macrophage inflammatory protein 2-alpha (Mip2-alpha), is a member of CXC chemokine family that binds to CXC chemokine receptor 2 (Cxcr2). Cxcl2 is mainly produced by macrophages, endothelial cells, epithelial cells and tumor cells. Cxcl2 plays important roles in various biological progresses such as angiogenesis, inflammation, immune response and cancer biological behaviors. Under inflammatory conditions in CNS, microglia and astrocytes may secrete Cxcl2 as well as other chemokines, which induces chemotaxis and infiltration of circulatory neutrophils. Indeed, previous studies suggest that Cxcl2 is involved in Alzheimer disease, multiple sclerosis and brain ischemia.


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