Mouse IFN-gamma ELISA Kit0 Publications

Catalog number: KE10001



96 T


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Product name:
Mouse IFN-gamma ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

5.5 pg/mL

31.25-2000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 92% 79%-104%
Mouse serum 91% 81%-95%

Samplenmean (pg/mL)SDCV%
1 20 207.1 10.3 5.0
2 20 367.4 16.5 4.5
3 20 1,101.3 76.1 6.9

Samplenmean (pg/mL)SDCV%
1 24 184.4 15.0 8.2
2 24 330.7 32.4 9.8
3 24 917.8 88.9 9.7

Product overview:
KE100001 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-γ ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-γ. The assay recognizes mouse IFN-γ. A Polyclonal antibody specific for IFN-γ has been pre-coated onto the microwells. The IFN-γ protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for IFN-γ is added to detect the captured IFN-γ protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Ifn, IFN g, IFN gamma, Ifng, IFN-gamma, interferon gamma

The IFNs were originally discovered as agents that interfere with viral replication. Initially, they were classified by the secreting cell type but are now classified into type I and type II according to receptor specificity and sequence homology. Interferon gamma (IFN-γ) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant Ifng expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

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