KE100001 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-γ ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-γ. The assay recognizes mouse IFN-γ. A Polyclonal antibody specific for IFN-γ has been pre-coated onto the microwells. The IFN-γ protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for IFN-γ is added to detect the captured IFN-γ protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.