Mouse IL10 ELISA Kit

Catalog number: KE10008

规格

单价

96 T

¥2600

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Overview


Product name:
Mouse IL10 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
2.5 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Serum 84% 77%-92%
Cell culture supernatant 104% 83%-124%

Product overview:
KE10008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL10. The assay recognizes mouse IL10. A monoclonal antibody specific for mouse IL10 has been pre-coated onto the microwells. The mouse IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse IL10 is added to detect the captured mouse IL10 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF
Background

Interleukin-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class IL antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.


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