Mouse IL10 ELISA Kit2 Publications

Catalog number: KE10008



96 T


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Product name:
Mouse IL10 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

2.5 pg/mL

15.6-1000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 104% 83%-124%
Mouse serum 84% 77%-92%

Samplenmean (pg/mL)SDCV%
1 20 1,002.3 39.8 4.0
2 20 280.0 6.8 2.4
3 20 78.2 5.1 6.5

Samplenmean (pg/mL)SDCV%
1 24 1,000.7 61.1 6.1
2 24 330.5 25.6 7.7
3 24 99.3 8.7 8.7

Product overview:
KE10008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL10. The assay recognizes mouse IL10. A monoclonal antibody specific for mouse IL10 has been pre-coated onto the microwells. The mouse IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse IL10 is added to detect the captured mouse IL10 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

CSIF, Il 10, Il10, IL-10, interleukin 10, Interleukin-10

Interleukin-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class IL antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

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