Mouse IL17A ELISA Kit0 Publications

Catalog number: KE10020



96 T


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Product name:
Mouse IL17A ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

2.6 pg/mL

7.8-500 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 93% 87%-105%
Mouse serum 93% 74%-112%

Samplenmean (pg/mL)SDCV%
1 20 9.9 0.8 8.0
2 20 104.5 4.5 4.3
3 20 386.5 26.1 6.8

Samplenmean (pg/mL)SDCV%
1 24 10.2 1.0 9.6
2 24 93.7 8.6 9.2
3 24 428.2 42.1 9.8

Product overview:
KE10020 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL17A ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL17A. The assay recognizes mouse IL17A. A monoclonal antibody specific for mouse IL17A has been pre-coated onto the microwells. The mouse IL17A protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL17A is added to detect the captured mouse IL17A protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Ctla 8, Ctla8, CTLA-8, Il17, IL-17, Il17a, IL-17A, interleukin 17A, Interleukin-17A

Il17a, also named as Il-17, is a proinflammatory cytokine. Il-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The Il-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. Il-17 and IL-22, are highly increased.

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