Mouse IL23 ELISA Kit

Catalog number: KE10013

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Overview


Product name:
Mouse IL23 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
3 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 89% 80%-100%
Cell culture supernatant 109% 84%-123%

Product overview:
K10013 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL23 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL23. The assay recognizes mouse IL23 complex which has two subunits IL23p19 and IL12p40. A monoclonal antibody specific for mouse IL23p19 has been pre-coated onto the microwells. The mouse IL23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL12p40 is added to detect the captured mouse IL23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Background

Interleukin 23 (il-23) is a member of the iL12 cytokine family and composed of two subunits, il12p40 and il23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for il-23 (the il-23 receptor) has been identified and is composed of Il-12Rβ1 and Il-23R. Il-23 is expressed chiefly by the macrophages and DCs. The il-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of il-23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). Il-23 acts also as an end-stage effector cytokine through direct action on macrophages.


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