Mouse IL5 ELISA Kit 0 Publications

Catalog number: KE10018



96 T


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Product name:
Mouse IL5 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

1.0 pg/mL

7.8-500 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 94% 82%-108%
Mouse serum 90% 76%-116%

Samplenmean (pg/mL)SDCV%
1 20 10.3 0.4 3.9
2 20 108.0 2.0 1.9
3 20 556.4 21.6 4.7

Samplenmean (pg/mL)SDCV%
1 24 10.4 0.5 4.9
2 24 111.4 1.8 1.6
3 24 503.0 17.4 3.5

Product overview:
KE10018 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL5. The assay recognizes mouse IL5. A monoclonal antibody specific for IL5 has been pre-coated onto the microwells. The IL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL5 is added to detect the captured IL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

B-cell growth factor II, BCGF-II, Il 5, Il5, IL-5, interleukin 5, Interleukin-5

Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. IL-5 is a key hematopoietic cytokine in eosinophil differentiation, maturation, recruitment and activation at sites of allergic inflammation. IL-5 also plays a role in the development, metabolism, and function of basophils. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an α chain that binds IL-5 with low affinity and a βchain that does not bind IL-5, but together with the IL-5α chain, constitutes the high affinity IL-5 receptor. The β chain is common to the IL-3, IL-5 and Gm-csf receptors and has been shown to signal through the JAK/Stat pathway.

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