Mouse IL6 ELISA Kit5 Publications

Catalog number: KE10007



96 T


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Product name:
Mouse IL6 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

3.8 pg/mL

15.6-1000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 108% 96%-123%
Mouse serum 99% 75%-119%

Samplenmean (pg/mL)SDCV%
1 20 133.3 6.1 4.5
2 20 204.3 7.6 3.7
3 20 778.1 52.7 6.8

Samplenmean (pg/mL)SDCV%
1 24 142.3 11.6 8.2
2 24 223.0 18.8 8.4
3 24 827.3 81.2 9.8

Product overview:
KE10007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL6. The assay recognizes mouse IL6. A monoclonal antibody specific for mouse IL6 has been pre-coated onto the microwells. The mouse IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse IL6 is added to detect the captured mouse IL6 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Il 6, Il6, IL-6, interleukin 6, Interleukin-6

Interleukin-6 (Il-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. Il-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. Il-6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Il-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. Il-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, Il-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

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