验证数据展示
经过测试的应用
| Positive WB detected in | |
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66360-1-Ig targets Mouse IgG1 isotype control in WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA applications and shows reactivity with megathura crenulata samples.
| 经测试应用 | WB, FC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, FC, IP, CoIP, RIP, Neutralization |
| 经测试反应性 | megathura crenulata |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Mouse IgG1 isotype control |
| 别名 | Mouse IgG1, Kappa light chain |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2827991 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Mouse IgG1 isotype control antibody 66360-1-Ig | Download protocol |
| FC protocol for Mouse IgG1 isotype control antibody 66360-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses | ||
FASEB J Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection | ||
J Immunol Phagocytic Plasma Cells in Teleost Fish Provide Insights into the Origin and Evolution of B Cells in Vertebrates | ||
Exp Cell Res CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway. | ||
Am J Rhinol Allergy Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis. | ||
bioRxiv Immunojanus Particles for low-volume and isolation-free unlabeled characterization of small Extracellular Vesicle in biofluids: Characterization of disease type by surface marker profiling |