Mouse MCP-1 ELISA Kit0 Publications

Catalog number: KE10006



96 T


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Product name:
Mouse MCP-1 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

1.3 pg/mL

3.13-200 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 102% 85%-119%
Mouse serum 91% 76%-127%

Samplenmean (pg/mL)SDCV%
1 20 196.2 17.4 8.8
2 20 52.3 2.9 5.5
3 20 11.2 0.7 6.6

Samplenmean (pg/mL)SDCV%
1 24 110.1 9.2 8.4
2 24 33.1 2.6 8.0
3 24 8.5 0.7 8.4

Product overview:
KE10006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous MCP-1. The assay recognizes mouse MCP-1. A monoclonal antibody specific for MCP-1 has been pre-coated onto the microwells. The MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for MCP-1 is added to detect the captured MCP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, MCP-1, Scya2, Sigje, SMC CF

Monocyte chemotactic protein 1 (Mcp1; also known as Ccl2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

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