Mouse VEGF ELISA Kit

Catalog number: KE10009

规格

单价

96 T

¥2600

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Overview


Product name:
Mouse VEGF ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
2 pg/mL

Range:
7.8-500 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Serum 93% 74%-110%
Cell culture supernatant 94% 92%-98%

Product overview:
KE10009 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse VEGF. The assay recognizes mouse VEGF. A polyclonal antibody specific for mouse VEGF has been pre-coated onto the microwells. The mouse VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse VEGF is added to detect the captured mouse VEGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
Background

Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.


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