验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HEK-293 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21679-1-AP targets NANOS3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NANOS3 fusion protein Ag16014 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nanos homolog 3 (Drosophila) |
| Synonyms | NOS-3, NOS3, NOS 3, NANOS1L, nanos homolog 3 (Drosophila) |
| Calculated Molecular Weight | 192 aa, 21 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC101209 |
| Gene Symbol | NANOS3 |
| Gene ID (NCBI) | 342977 |
| RRID | AB_10859250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60323 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The NANOS gene family is required for germ cell development in diverse organisms. NANOS3 is found in migrating primordial germ cells, and the homozygous deficiency of NANOS3 in the mouse results in the complete loss of germ cells in both sexes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NANOS3 antibody 21679-1-AP | Download protocol |
| IHC protocol for NANOS3 antibody 21679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Regulation of both transcription and RNA turnover contribute to germline specification. | ||
Environ Pollut Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling | ||
J Pathol The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition. | ||
Exp Cell Res Pum2 mediates Sirt1 mRNA decay and exacerbates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. | ||
J Ethnopharmacol Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC (soluble guanylate cyclase) - cGMP (cyclic guanosine monophosphate) - PKG (protein kinase G) pathway |