验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HepG2 cells, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12995-1-AP targets NEU4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NEU4 fusion protein Ag3657 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sialidase 4 |
| 别名 | N acetyl alpha neuraminidase 4, NEU4, sialidase 4 |
| 计算分子量 | 496 aa, 53 kDa |
| 观测分子量 | 52-55 kDa |
| GenBank蛋白编号 | BC012899 |
| 基因名称 | NEU4 |
| Gene ID (NCBI) | 129807 |
| RRID | AB_2149206 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WWR8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEU4, also named as LP5125, belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. It may function in lysosomal catabolism of sialylated glycoconjugates. NEU4 has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEU4 antibody 12995-1-AP | Download protocol |
| IHC protocol for NEU4 antibody 12995-1-AP | Download protocol |
| IP protocol for NEU4 antibody 12995-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles. | ||
Cell Signal A novel epidermal growth factor receptor-signaling platform and its targeted translation in pancreatic cancer. | ||
Cell Signal Neu1 sialidase and matrix metalloproteinase-9 cross-talk is essential for neurotrophin activation of Trk receptors and cellular signaling. | ||
Glycoconj J Dependence of pathogen molecule-induced toll-like receptor activation and cell function on Neu1 sialidase. | ||
Glycoconj J Thymoquinone from nutraceutical black cumin oil activates Neu4 sialidase in live macrophage, dendritic, and normal and type I sialidosis human fibroblast cells via GPCR Galphai proteins and matrix metalloproteinase-9. |