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验证数据展示

  • IF Staining of HepG2 using 16396-1-AP

    IF Staining of HepG2 using 16396-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 16396-1-AP

    WB analysis using 16396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16396-1-AP

    WB analysis using 16396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16396-1-AP

    IP experiment of HeLa using 16396-1-AP

    IP result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 4ug; Detection:16396-1-AP 1:6000) with HeLa cells lysate 1520 ug.

  • IHC staining of human pancreas cancer using 16396-1-AP

    IHC staining of human pancreas cancer using 16396-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IF Staining of HepG2 using 16396-1-AP

    IF Staining of HepG2 using 16396-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (16396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 16396-1-AP

    IHC staining of human liver cancer using 16396-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • Featured Product
  • KD/KO Validated

NRF2, NFE2L2 Polyclonal antibody

NRF2, NFE2L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16396-1-AP
Publications(1888)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Synonyms

NRF2, NFE2L2, HEBP1, Nrf 2, NFE2-related factor 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, DMSO treated HeLa cells, A549 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human breast cancer tissue, human kidney tissue, human pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMG132 treated HepG2 cells, HepG2 cells

This antibody is only recommended for WB detection of human samples

Planning an IHC experiment? We recommend our IHCeasy NFE2L2/NRF2 Ready-To-Use IHC Kit. NFE2L2/NRF2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16396-1-AP targets NRF2, NFE2L2 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, chIP
Tested Reactivity human
Cited Reactivityhuman, rabbit, monkey, chicken, goat, yellow catfish, seiurus aurocapilla, ducks
Immunogen NRF2, NFE2L2 fusion protein Ag9489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor (erythroid-derived 2)-like 2
Synonyms NRF2, NFE2L2, HEBP1, Nrf 2, NFE2-related factor 2
Calculated Molecular Weight 605 aa, 68 kDa
Observed Molecular Weight 110 kDa, 68 kDa
GenBank Accession NumberBC011558
Gene Symbol NRF2
Gene ID (NCBI) 4780
RRIDAB_2782956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16236
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

实验方案

Product Specific Protocols
WB protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IHC protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IF protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IP protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
View Article
humanWB

Nature

Structure of the nutrient-sensing hub GATOR2.

Authors - Max L Valenstein
View Article
humanWB

Gastroenterology

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang
View Article
humanWB, IP

Cancer Cell

iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.

Authors - Wenjie Ge
  • KD Validated
View Article
human

Cell Metab

Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.

Authors - Jiajun Zhu
View Article
mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
View Article