验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11399-1-AP targets NMNAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NMNAT1 fusion protein Ag1944 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nicotinamide nucleotide adenylyltransferase 1 |
| 别名 | NMN/NaMN adenylyltransferase 1, Nicotinate-nucleotide adenylyltransferase 1, Nicotinamide-nucleotide adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NaMN adenylyltransferase 1 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 28-32 kDa |
| GenBank蛋白编号 | BC014943 |
| 基因名称 | NMNAT1 |
| Gene ID (NCBI) | 64802 |
| RRID | AB_10699868 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAN9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NMNAT1 antibody 11399-1-AP | Download protocol |
| IHC protocol for NMNAT1 antibody 11399-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol Pre-emptive Short-term Nicotinamide Mononucleotide Treatment in a Mouse Model of Diabetic Nephropathy. | ||
Metabolism Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice. | ||
J Proteome Res Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD(+) and Suppresses Glycolysis, the TCA Cycle, and Cell Growth | ||
Sci Rep Nicotinamide mononucleotide ameliorates adriamycin-induced renal damage by epigenetically suppressing the NMN/NAD consumers mediated by Twist2 | ||
Food Funct β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice | ||
EMBO J Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function |