验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human cerebellum tissue, human stomach tissue, mouse spinal cord tissue, pig cerebellum tissue, rat brain tissue, mouse cerebellum tissue |
| Positive IP detected in | mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13129-1-AP targets TrkB in WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, Peptide array |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, rabbit, zebrafish |
| 免疫原 | TrkB fusion protein Ag3790 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neurotrophic tyrosine kinase, receptor, type 2 |
| 别名 | NTRK2, Tkrb, NTRK 2, GP145-TrkB, GP145 TrkB |
| 计算分子量 | 822 aa, 92 kDa |
| 观测分子量 | 90-95 kDa, 140-145 kDa |
| GenBank蛋白编号 | BC031835 |
| 基因名称 | TrkB |
| Gene ID (NCBI) | 4915 |
| RRID | AB_2155156 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16620 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TrkB antibody 13129-1-AP | Download protocol |
| IHC protocol for TrkB antibody 13129-1-AP | Download protocol |
| IF protocol for TrkB antibody 13129-1-AP | Download protocol |
| IP protocol for TrkB antibody 13129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Chemosphere Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny. | ||
Oxid Med Cell Longev Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway. | ||
J Affect Disord Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel | ||
ACS Chem Neurosci Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine. | ||
Am J Cancer Res LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance. | ||
Cell Biosci A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation. |
