验证数据展示

WB analysis using 10837-1-AP
various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 10837-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OPTN (10837-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP experiment of mouse brain using 10837-1-AP
IP result of anti-OPTN (IP:10837-1-AP, 3ug; Detection:10837-1-AP 1:800) with mouse brain tissue lysate 8000ug.
IHC staining of human gliomas using 10837-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human gliomas using 10837-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 40x lens).
WB analysis of HeLa using 10837-1-AP
WB result of OPTN antibody (10837-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPTN transfected HeLa cells.
IF Staining of rat eye using 10837-1-AP
Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using OPTN antibody (10837-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10837-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (10837-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

OPTN/Optineurin Polyclonal antibody

OPTN/Optineurin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 10837-1-AP

Publications(111)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

Synonyms

Optineurin, OPTN, FIP-2, FIP2, FIP 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (111)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat eye tissue
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 13 publications below
WB	See 93 publications below
IHC	See 5 publications below
IF	See 20 publications below
IP	See 6 publications below
CoIP	See 2 publications below

产品信息

10837-1-AP targets OPTN/Optineurin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, hamster
Immunogen	OPTN/Optineurin fusion protein Ag1272 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	optineurin
Synonyms	Optineurin, OPTN, FIP-2, FIP2, FIP 2
Calculated Molecular Weight	66 kDa
Observed Molecular Weight	66-70 kDa
GenBank Accession Number	BC013876
Gene Symbol	OPTN
Gene ID (NCBI)	10133
RRID	AB_2156665
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96CV9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol
IHC protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol
IF protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol
IP protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nature The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Authors - Michael Lazarou View Article
mouse	WB	Cell NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria. Authors - Zhenyu Zhong View Article
human	CoIP	Mol Cell TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1 Authors - François Le Guerroué View Article
human	IF	Acta Neuropathol p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS. Authors - Safa Al-Sarraj View Article
human	WB	Mol Cell Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain. Authors - Shireen A Sarraf KO Validated View Article
human	WB	Mol Cell Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. Authors - Susanne Zellner View Article

验证数据展示

WB analysis using 10837-1-AP

FC experiment of HeLa using 10837-1-AP

IP experiment of mouse brain using 10837-1-AP

IHC staining of human gliomas using 10837-1-AP

IHC staining of human gliomas using 10837-1-AP

WB analysis of HeLa using 10837-1-AP

IF Staining of rat eye using 10837-1-AP

IF Staining of MCF-7 using 10837-1-AP

OPTN/Optineurin Polyclonal antibody

OPTN/Optineurin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1

p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS.

Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.