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Biotin-conjugated P-cadherin Polyclonal antibody

P-cadherin Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-13773

Print datasheet

Synonyms

P cadherin, HJMD, CDHP, CDH3, Cadherin-3

验证数据展示

  • IHC staining of human breast cancer using Biotin-13773

    IHC staining of human breast cancer using Biotin-13773

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-13773 (P-cadherin antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using Biotin-13773

    IHC staining of human breast cancer using Biotin-13773

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-13773 (P-cadherin antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

Biotin-13773 targets P-cadherin in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen P-cadherin fusion protein Ag4758 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cadherin 3, type 1, P-cadherin (placental)
Synonyms P cadherin, HJMD, CDHP, CDH3, Cadherin-3
Calculated Molecular Weight 829 aa, 91 kDa
GenBank Accession NumberBC041846
Gene Symbol P-cadherin
Gene ID (NCBI) 1001
RRIDAB_2883057
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22223
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

实验方案

Product Specific Protocols
IHC protocol for Biotin P-cadherin antibody Biotin-13773Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Invest Dermatol

Lgr4 Deletion Delays the Hair Cycle and Inhibits the Activation of Hair Follicle Stem Cells.

Authors - Xiaolin Ren
View Article