验证数据展示

WB analysis using 25614-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 25614-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25614-1-AP
WB result of P27; KIP1 antibody (25614-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P27; KIP1 transfected HeLa cells.
IHC staining of human breast cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CDKN1B antibody included
IHC staining of human breast cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CDKN1B antibody included
IP experiment of NIH/3T3 using 25614-1-AP
IP result of anti-P27; KIP1 (IP:25614-1-AP, 4ug; Detection:25614-1-AP 1:1000) with NIH/3T3 cells lysate 3440 ug.
IF Staining of mouse testis using 25614-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using P27; KIP1 antibody (25614-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 25614-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using P27; KIP1 antibody (25614-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of MCF-7 using 25614-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human P27; KIP1 (25614-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
IF Staining of HepG2 using 25614-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P27; KIP1 antibody (25614-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

Featured Product
KD/KO验证

P27; KIP1 Polyclonal antibody

P27; KIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 25614-1-AP

产品说明书

引用文献(214)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (4)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

别名

CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (214)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, NIH/3T3 cells, HepG2 cells, MCF-7 cells, Jurkat cells
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue, human colon cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy CDKN1B Ready-To-Use IHC Kit. CDKN1B primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 207 publications below
IHC	See 16 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 1 publications below

产品信息

25614-1-AP targets P27; KIP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat, canine, bovine, sheep
免疫原	P27; KIP1 fusion protein Ag22582 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cyclin-dependent kinase inhibitor 1B (p27, Kip1)
别名	CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27
计算分子量	198 aa, 22 kDa
观测分子量	27 kDa
GenBank蛋白编号	BC001971
基因名称	p27 Kip1/CDKN1B
Gene ID (NCBI)	1027
RRID	AB_2880161
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P46527
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

实验方案

Product Specific Protocols
WB protocol for P27; KIP1 antibody 25614-1-AP	Download protocol
IHC protocol for P27; KIP1 antibody 25614-1-AP	Download protocol
IF protocol for P27; KIP1 antibody 25614-1-AP	Download protocol
IP protocol for P27; KIP1 antibody 25614-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IHC	Gastroenterology Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6. Authors - Yuhong Luo View Article
human	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
human	WB	Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Authors - Xi Jin View Article
human	WB	Mol Cancer CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. Authors - Lianyong Liu View Article
human	WB	Small Synergistic Silencing of Skp2 by siRNA Self-Assembled Nanoparticles as a Therapeutic Strategy for Advanced Prostate Cancer. Authors - Hong Liang View Article
human	WB	Cell Death Dis UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer Authors - Xiaoling Hong View Article

验证数据展示

WB analysis using 25614-1-AP

WB analysis using 25614-1-AP

WB analysis of HeLa using 25614-1-AP

IHC staining of human breast cancer using 25614-1-AP

IHC staining of human breast cancer using 25614-1-AP

IP experiment of NIH/3T3 using 25614-1-AP

IF Staining of mouse testis using 25614-1-AP

IF Staining of mouse testis using 25614-1-AP

FC experiment of MCF-7 using 25614-1-AP

IF Staining of HepG2 using 25614-1-AP

P27; KIP1 Polyclonal antibody

P27; KIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma.

Synergistic Silencing of Skp2 by siRNA Self-Assembled Nanoparticles as a Therapeutic Strategy for Advanced Prostate Cancer.

UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer