验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, Raji cells, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
产品信息
13539-1-AP targets PAK6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Immunogen | PAK6 fusion protein Ag4467 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | p21 protein (Cdc42/Rac)-activated kinase 6 |
| Synonyms | PAK5, PAK 6, PAK 5, p21-activated kinase 6, p21 activated kinase 6 |
| Calculated Molecular Weight | 681 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC035596 |
| Gene Symbol | PAK6 |
| Gene ID (NCBI) | 56924 |
| RRID | AB_2158467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the C-terminal of PAK6. This antibody has weak cross-reaction with other PAK members.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAK6 antibody 13539-1-AP | Download protocol |
| IHC protocol for PAK6 antibody 13539-1-AP | Download protocol |
| IF protocol for PAK6 antibody 13539-1-AP | Download protocol |
| IP protocol for PAK6 antibody 13539-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncol Lett PAK6 promotes cervical cancer progression through activation of the Wnt/β-catenin signaling pathway.
| ||
J Pain Res Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation. | ||
BMC Nephrol Urinary exosome proteins PAK6 and EGFR as noninvasive diagnostic biomarkers of diabetic nephropathy |
