验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U2OS cells, SH-SY5Y cells |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IHC | See 1 publications below |
产品信息
55149-1-AP targets PARP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | poly (ADP-ribose) polymerase 2 |
| 别名 | ADPRT 2, ADPRT2, ADPRTL2, ADPRTL3, hPARP 2, pADPRT 2, PARP 2, PARP2, poly (ADP ribose) polymerase 2, Poly [ADP ribose] polymerase 2, Poly[ADP ribose] synthase 2 |
| 计算分子量 | 66 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | NM_005484 |
| 基因名称 | PARP2 |
| Gene ID (NCBI) | 10038 |
| RRID | AB_10858796 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UGN5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PARP2 antibody 55149-1-AP | Download protocol |
| IF protocol for PARP2 antibody 55149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma. | ||
Cells Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development | ||
Eur J Med Chem Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer. | ||
EMBO J Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function | ||
Eur J Med Chem Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation |