PD-1/CD279 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66220-1-Ig |CloneNo.: 4H4D1

  • Print page
  • Download PDF

Species specificity:
human, rat, mouse

Positive WB detected in:
Raji cells, human lymph node tissue, Jurkat cells, mouse thymus tissue, Ramos cells, rat spleen tissue, RAW 264.7 cells

Positive IHC detected in:
human tonsillitis tissue, mouse spleen tissue, human lymphoma tissue

Positive IF detected in:
human tonsillitis tissue

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:400
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
programmed cell death 1

Calculated molecular weight:
288aa,32 kDa

Observed molecular weight:
32 kDa, 47-55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PDCD1

Synonyms
CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2
Background

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).


Back
to top