验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HEK293 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66119-1-Ig targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | PDH E1 Alpha fusion protein Ag12556 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | pyruvate dehydrogenase (lipoamide) alpha 1 |
| 别名 | PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC002406 |
| 基因名称 | PDHA1 |
| Gene ID (NCBI) | 5160 |
| RRID | AB_2881518 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08559 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| IHC protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| IF protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| IP protocol for PDH E1 Alpha antibody 66119-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
J Cell Biol PINK1 disables the anti-fission machinery to segregate damaged mitochondria for mitophagy. | ||
Cell Rep A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria. | ||
Toxicol Appl Pharmacol Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis. | ||
Immunobiology The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus. | ||
Nat Metab MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1α promotes tumour immune evasion.
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