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  • KD/KO Validated

PEX1 Polyclonal antibody

PEX1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13669-1-AP

Print datasheet

Synonyms

Peroxin 1, Peroxisome biogenesis factor 1, PEX1, ZWS1



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A431 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13669-1-AP targets PEX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PEX1 fusion protein Ag4623 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxisomal biogenesis factor 1
Synonyms Peroxin 1, Peroxisome biogenesis factor 1, PEX1, ZWS1
Calculated Molecular Weight 1283 aa, 143 kDa
Observed Molecular Weight 143 kDa
GenBank Accession NumberBC035575
Gene Symbol PEX1
Gene ID (NCBI) 5189
RRIDAB_2162104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEX1 antibody 13669-1-APDownload protocol
IHC protocol for PEX1 antibody 13669-1-APDownload protocol
IF protocol for PEX1 antibody 13669-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Autophagy

Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.

Authors - Marcus Nordgren
humanWB,IF

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
humanWB

Autophagy

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Authors - Zhenhong Ni
humanIHC

Eur J Hum Genet

Spectrum of PEX1 and PEX6 variants in Heimler syndrome.

Authors - Claire E L Smith
humanWB

Mol Ther Methods Clin Dev

AAV-mediated PEX1 gene augmentation improves visual function in the PEX1-Gly844Asp mouse model for mild Zellweger spectrum disorder.

Authors - Catherine Argyriou
mouseWB,IF

Exp Eye Res

A longitudinal study of retinopathy in the PEX1-Gly844Asp mouse model for mild Zellweger Spectrum Disorder.

Authors - Catherine Argyriou