验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, BxPC-3 cells, human heart tissue, NIH/3T3 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, Jurkat cells, MCF-7 cells, Raji cells |
| Positive IP detected in | Jurkat cells, mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human lung cancer tissue, human heart tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13389-1-AP targets PFKP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PFKP fusion protein Ag4047 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphofructokinase, platelet |
| 别名 | PFKF, PFK-C, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type |
| 计算分子量 | 784 aa, 86 kDa |
| 观测分子量 | 80-86 kDa |
| GenBank蛋白编号 | BC029138 |
| 基因名称 | PFKP |
| Gene ID (NCBI) | 5214 |
| RRID | AB_2252278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01813 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PFKP antibody 13389-1-AP | Download protocol |
| IHC protocol for PFKP antibody 13389-1-AP | Download protocol |
| IF protocol for PFKP antibody 13389-1-AP | Download protocol |
| IP protocol for PFKP antibody 13389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Mol Cell R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis.
| ||
Stem Cell Reports The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway. | ||
Acta Pharmacol Sin Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. |
