验证数据展示
经过测试的应用
| Positive WB detected in | pig spinal cord tissue, human brain tissue, mouse testis tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12717-1-AP targets PMP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | PMP2 fusion protein Ag3411 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peripheral myelin protein 2 |
| 别名 | FABP8, M FABP, MP2, Myelin P2 protein, P2, peripheral myelin protein 2, PMP2 |
| 计算分子量 | 132 aa, 15 kDa |
| 观测分子量 | 15-20 kDa |
| GenBank蛋白编号 | BC034997 |
| 基因名称 | PMP2 |
| Gene ID (NCBI) | 5375 |
| RRID | AB_2166978 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02689 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PMP2 (peripheral myelin protein-2, also known as P2) is one of the major proteins of peripheral myelin and appears to be related to the transport of fatty acids or the metabolism of myelin lipids.P2 constitutes up to 15% of total protein of peripheral nervous system (PNS) myelin. It is also present in small amounts in central nervous system (CNS) myelin, being abundant in spinal cord and brain stem myelin. As a structural protein, P2 is thought to stabilize the myelin membranes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PMP2 antibody 12717-1-AP | Download protocol |
| IHC protocol for PMP2 antibody 12717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes. | ||
Nat Neurosci Disentangling glial diversity in peripheral nerves at single-nuclei resolution. | ||
Neuron Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction. | ||
J Cell Biol Fatty acid-binding proteins 3, 7, and 8 bind cholesterol and facilitate its egress from lysosomes
| ||
Elife Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. |
