POMC Monoclonal antibody

POMC Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C12C7

Cat No : 66358-1-Ig

Print datasheet

Synonyms

ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin



经过测试的应用

Positive WB detected inpig brain tissue, rat brain tissue, A375 cells, human placenta tissue, PC-12 cells, Transfected HEK-293 cells
Positive IHC detected inhuman pituitary tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66358-1-Ig targets POMC in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, rat, mouse, pig
Cited Reactivityhuman, mouse, rat, pig
Immunogen POMC fusion protein Ag17311 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proopiomelanocortin
Synonyms ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin
Calculated Molecular Weight 267 aa, 29 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC065832
Gene Symbol POMC
Gene ID (NCBI) 5443
RRIDAB_2881737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01189
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

实验方案

Product Specific Protocols
WB protocol for POMC antibody 66358-1-IgDownload protocol
IHC protocol for POMC antibody 66358-1-IgDownload protocol
IF protocol for POMC antibody 66358-1-IgDownload protocol
FC protocol for POMC antibody 66358-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Elife

Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.

Authors - Xuebin Zhang
mouseIF

Biomed Pharmacother

919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities.

Authors - Jing-Wei Xing
ratWB,IF

Sci Rep

Gold nanoclusters eliminate obesity induced by antipsychotics.

Authors - Meng He
mouseWB,IF

Biochem Biophys Res Commun

Elongator regulates the melanocortin satiety pathway.

Authors - Joseph Walters
ratIF

Reprod Sci

FoxO1 Regulates Neuropeptide Y and Pro-opiomelanocortin in the Hypothalamus of Rat Offspring Small for Gestational Age.

Authors - Lifang Zhang
pigWB,IHC

Food Addit Contam Part A Chem Anal

FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs.

Authors - Quancheng Liu