POMC Monoclonal antibody
POMC Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse, pig
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3C12C7
验证数据展示
经过测试的应用
Positive WB detected in | pig brain tissue, rat brain tissue, A375 cells, human placenta tissue, PC-12 cells, Transfected HEK-293 cells |
Positive IHC detected in | human pituitary tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66358-1-Ig targets POMC in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | human, rat, mouse, pig |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | POMC fusion protein Ag17311 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | proopiomelanocortin |
Synonyms | ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin |
Calculated Molecular Weight | 267 aa, 29 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC065832 |
Gene Symbol | POMC |
Gene ID (NCBI) | 5443 |
RRID | AB_2881737 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P01189 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for POMC antibody 66358-1-Ig | Download protocol |
IHC protocol for POMC antibody 66358-1-Ig | Download protocol |
IF protocol for POMC antibody 66358-1-Ig | Download protocol |
FC protocol for POMC antibody 66358-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Elife Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma. | ||
Biomed Pharmacother 919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities. | ||
Reprod Sci FoxO1 Regulates Neuropeptide Y and Pro-opiomelanocortin in the Hypothalamus of Rat Offspring Small for Gestational Age. | ||
Food Addit Contam Part A Chem Anal FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs. |