验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, fetal human brain tissue, pig brain tissue, zebrafish tissue, T-47D cells, Jurkat cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67070-1-Ig targets PPP1CA in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, zebrafish, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | Human, mouse, rat, pig, zebrafish, rabbit |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PPP1CA fusion protein Ag28451 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, catalytic subunit, alpha isoform |
| 别名 | PP 1A, PPP1A, PPP1CA |
| 计算分子量 | 330 aa, 38 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC001888 |
| 基因名称 | PPP1CA |
| Gene ID (NCBI) | 5499 |
| RRID | AB_2882379 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62136 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1CA antibody 67070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration
| ||
J Neurosci CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease. | ||
Neurotoxicology Nuclear accumulation of histone deacetylase 4 (HDAC4) by PP1-mediated dephosphorylation exerts neurotoxicity in Pb-exposed neural cells. | ||
Exp Ther Med Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation. | ||
Zool Res EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50
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