验证数据展示
经过测试的应用
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
10766-1-AP targets PRG2 in IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, ELISA Application Description |
| Cited Applications | IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | PRG2 fusion protein Ag1150 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein) |
| Synonyms | BMPG, Bone marrow proteoglycan, EMBP, MBP, MBP1, PRG2, Proteoglycan 2 |
| Calculated Molecular Weight | 25 kDa |
| GenBank Accession Number | BC005929 |
| Gene Symbol | PRG2 |
| Gene ID (NCBI) | 5553 |
| RRID | AB_2169223 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13727 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRG2, also known as BMPG, has 222 amino acid residues with a 16 N-terminal signal sequence, so the mature form has 206 amino acid residues. High levels of PRG2 are present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein can be cleaved into eosinophil granule major basic protein (MBP), which is the major constituent of the crystalline core of the eosinophil granule. MBP is highly basic and toxic to both mammalian cells and parasites, and it functions in the defense against invading parasites (PMID: 21270431).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PRG2 antibody 10766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Allergy Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity | ||
Exp Eye Res Role of TGF-β in tissue eosinophilia associated with vernal keratoconjunctivitis. |
