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验证数据展示

  • WB analysis using 66429-1-Ig

    WB analysis using 66429-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66429-1-Ig (PKC gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 66429-1-Ig

    IHC staining of human brain using 66429-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 66429-1-Ig

    IHC staining of human brain using 66429-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 66429-1-Ig

    IF Staining of SH-SY5Y using 66429-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66429-1-Ig(PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of SH-SY5Y using 66429-1-Ig

    FC experiment of SH-SY5Y using 66429-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (66429-1-Ig, Clone:2F4B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PKC Gamma Monoclonal antibody

PKC Gamma Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66429-1-Ig

产品说明书

CloneNo. 2F4B9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

PRKCG, PKC-gamma, PKCG, PKCC, EC:2.7.11.13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, human cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66429-1-Ig targets PKC Gamma in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PKC Gamma fusion protein Ag5910 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase C, gamma
别名 PRKCG, PKC-gamma, PKCG, PKCC, EC:2.7.11.13
计算分子量 78 kDa
观测分子量 78 kDa
GenBank蛋白编号BC047876
基因名称 PKC Gamma
Gene ID (NCBI) 5582
RRIDAB_2881800
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05129
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

实验方案

Product Specific Protocols
WB protocol for PKC Gamma antibody 66429-1-IgDownload protocol
IHC protocol for PKC Gamma antibody 66429-1-IgDownload protocol
IF protocol for PKC Gamma antibody 66429-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols