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PROCR Monoclonal antibody

PROCR Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1F5C4

Cat no : 67658-1-Ig

Print datasheet

Synonyms

Activated protein C receptor, APC receptor, bA42O4.2, CCCA, CCD41, CD201, Endothelial protein C receptor, EPCR, PROCR

验证数据展示

  • WB analysis using 67658-1-Ig

    WB analysis using 67658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67658-1-Ig

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67658-1-Ig

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman placenta tissue, pig spleen tissue, pig heart tissue, pig vein tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67658-1-Ig targets PROCR in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human, Pig
Cited Reactivityhuman, mouse
Immunogen PROCR fusion protein Ag25628 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein C receptor, endothelial (EPCR)
Synonyms Activated protein C receptor, APC receptor, bA42O4.2, CCCA, CCD41, CD201, Endothelial protein C receptor, EPCR, PROCR
Calculated Molecular Weight 238 aa, 27 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC014451
Gene Symbol PROCR
Gene ID (NCBI) 10544
RRIDAB_2882855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PROCR antibody 67658-1-IgDownload protocol
IHC protocol for PROCR antibody 67658-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Signal Transduct Target Ther

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.

Authors - Panpan Zhang
View Article