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PSD95-Specific,DLG4 Polyclonal antibody

PSD95-Specific,DLG4 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20665-1-AP

Print datasheet

Synonyms

Disks large homolog 4, DLG4, PSD 95, PSD95, PSD95-Specific,DLG4, SAP 90, SAP90, Synapse associated protein 90



经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inNeuro-2a cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20665-1-AP targets PSD95-Specific,DLG4 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications CoIP, FC, IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, hamster
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name discs, large homolog 4 (Drosophila)
Synonyms Disks large homolog 4, DLG4, PSD 95, PSD95, PSD95-Specific,DLG4, SAP 90, SAP90, Synapse associated protein 90
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberNM_001365
Gene Symbol DLG4
Gene ID (NCBI) 1742
RRIDAB_2687961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

实验方案

Product Specific Protocols
WB protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
IHC protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nat Neurosci

Foxp2 controls synaptic wiring of corticostriatal circuits and vocal communication by opposing Mef2c.

Authors - Yi-Chuan Chen
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouseWB,IF

Neuron

Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.

Authors - Chao Wang
mouseWB

Sci Adv

Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission

Authors - Wenquan Liang
mouseWB

Sci Adv

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission

Authors - Pengcheng Ma
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin