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PSEN2-Specific Polyclonal antibody

PSEN2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16168-1-AP

Print datasheet

Synonyms

AD3L, AD3LP, AD4, AD5, E5 1, Presenilin 2, PS 2, PS2, PSEN2, PSEN2-Specific, PSNL2, STM 2, STM2

验证数据展示

  • WB analysis using 16168-1-AP

    WB analysis using 16168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 16168-1-AP

    WB analysis of A549 using 16168-1-AP

    WB result of PSEN2-Specific antibody (16168-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSEN2-Specific transfected A549 cells.

  • IHC staining of human kidney using 16168-1-AP

    IHC staining of human kidney using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 16168-1-AP

    IHC staining of human kidney using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inC6 cells, A549 cells, NIH/3T3 cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human colon tissue, mouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:800
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16168-1-AP targets PSEN2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name presenilin 2 (Alzheimer disease 4)
Synonyms AD3L, AD3LP, AD4, AD5, E5 1, Presenilin 2, PS 2, PS2, PSEN2, PSEN2-Specific, PSNL2, STM 2, STM2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberNM_000447
Gene Symbol PSEN2
Gene ID (NCBI) 5664
RRIDAB_2170748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Presenilins can regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. These transmembrane proteins are considered to be involved in early onset of Alzheimer's disease. Presenilin 2 mainly localizes in large neurons. It exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (about 23 kDa) fragments.This antibody is specific to presenilin 2. It will not bind presenilin 1.

实验方案

Product Specific Protocols
WB protocol for PSEN2-Specific antibody 16168-1-APDownload protocol
IHC protocol for PSEN2-Specific antibody 16168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Gut Microbes

Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis

Authors - Jinghua Jin
View Article
mouseWB

Neural Regen Res

MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

Authors - Bhupender Sharma
View Article