验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human kidney tissue, human plasma, human heart tissue, human colon tissue, PC-3 cells, human urine tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IHC | See 4 publications below |
产品信息
10754-2-AP targets PTGDS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | PTGDS fusion protein Ag1163 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prostaglandin D2 synthase 21kDa (brain) |
| 别名 | LPGDS, Lipocalin-type prostaglandin-D synthase, Glutathione-independent PGD synthase, EC:5.3.99.2, Cerebrin-28 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC005939 |
| 基因名称 | PTGDS |
| Gene ID (NCBI) | 5730 |
| RRID | AB_2174610 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41222 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycoprotein prostaglandin D2 synthase (PTGDS), also known as L-PGDS, PGDS, and LPGDS, is a member of the lipocalin superfamily and plays dual roles in prostaglandins metabolism and lipid transport (PMID: 34743203). PTGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PTGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. PTGDS is abundantly expressed in the brain and heart and has also been found in testis, prostate, kidney, spleen and leukocytes. It can be found as a collection of monomeric glycoforms with molecular weights in the 27-34 kDa range (PMID: 32430846).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTGDS antibody 10754-2-AP | Download protocol |
| IHC protocol for PTGDS antibody 10754-2-AP | Download protocol |
| IP protocol for PTGDS antibody 10754-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway. | ||
Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. | ||
Cell Cycle MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling. | ||
Cancer Manag Res Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation. | ||
Discov Oncol Transcriptomic analysis reveals potential crosstalk genes and immune relationship between triple-negative breast cancer and depression | ||
Med Sci Monit Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma. |
