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验证数据展示

  • WB analysis using 18053-1-AP

    WB analysis using 18053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 18053-1-AP

    IHC staining of human placenta using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 18053-1-AP

    IHC staining of human placenta using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 18053-1-AP

    WB analysis of HeLa using 18053-1-AP

    WB result of PTPN14 antibody (18053-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN14 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

PTPN14 Polyclonal antibody

PTPN14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18053-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

PEZ, PTP36, PTPN14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18053-1-AP targets PTPN14 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性canine
免疫原 PTPN14 fusion protein Ag12571 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 14
别名 PEZ, PTP36, PTPN14
计算分子量 1187 aa, 135 kDa
观测分子量 150 kDa
GenBank蛋白编号BC101754
基因名称 PTPN14
Gene ID (NCBI) 5784
RRIDAB_2284584
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

实验方案

Product Specific Protocols
WB protocol for PTPN14 antibody 18053-1-APDownload protocol
IHC protocol for PTPN14 antibody 18053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineWB

Biologicals

CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells

Authors - Zhenbin Liu
View Article