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验证数据展示

  • WB analysis using 66688-1-Ig

    WB analysis using 66688-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66688-1-Ig

    IHC staining of mouse brain using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66688-1-Ig

    IHC staining of mouse brain using 66688-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 66688-1-Ig

    WB analysis of HeLa using 66688-1-Ig

    WB result of RAB3GAP1 antibody (66688-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HeLa cells.

  • IF Staining of HEK-293 using 66688-1-Ig

    IF Staining of HEK-293 using 66688-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAB3GAP1 antibody (66688-1-Ig, Clone: 4A3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

RAB3GAP1 Monoclonal antibody

RAB3GAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66688-1-Ig

产品说明书

CloneNo. 4A3A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp434A012, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66688-1-Ig targets RAB3GAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 RAB3GAP1 fusion protein Ag17710 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB3 GTPase activating protein subunit 1 (catalytic)
别名 DKFZp434A012, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1
计算分子量 981 aa, 111 kDa
观测分子量 130 kDa
GenBank蛋白编号BC146809
基因名称 RAB3GAP1
Gene ID (NCBI) 22930
RRIDAB_2882042
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
IHC protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
IF protocol for RAB3GAP1 antibody 66688-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols