验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66339-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66339 targets RAB5A in IF/ICC applications and shows reactivity with human, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | RAB5A fusion protein Ag16781 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RAB5A, member RAS oncogene family |
| 别名 | RAB5, RAB5A, Ras related protein Rab 5A |
| 计算分子量 | 215 aa, 24 kDa |
| GenBank蛋白编号 | BC001267 |
| 基因名称 | RAB5A |
| Gene ID (NCBI) | 5868 |
| RRID | AB_2883562 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20339 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 RAB5A antibody CL594-66339 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |