验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
12121-1-AP targets RAD23B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat, pig |
| 免疫原 | RAD23B fusion protein Ag2763 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAD23 homolog B (S. cerevisiae) |
| 别名 | XP-C repair-complementing complex 58 kDa protein, p58, HR23B, HHR23B |
| 计算分子量 | 409 aa, 43 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC020973 |
| 基因名称 | RAD23B |
| Gene ID (NCBI) | 5887 |
| RRID | AB_10641048 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54727 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B displays aberrant mobility in SDS-polyacrylamide gels, as it is typically detected as a 58- to 60-kDa protein despite a predicted size of 42 kDa (PMID: 8692695, 12052895).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD23B antibody 12121-1-AP | Download protocol |
| IHC protocol for RAD23B antibody 12121-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB. | ||
Mol Cell Proteomics The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators | ||
Int J Biol Sci Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B. | ||
PLoS One Proteins with Intrinsically Disordered Domains Are Preferentially Recruited to Polyglutamine Aggregates. | ||
