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  • Featured Product
  • KD/KO Validated

RNF128 Polyclonal antibody

RNF128 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26015-1-AP

Print datasheet

Synonyms

GRAIL, ring finger protein 128, RNF128

验证数据展示

  • WB analysis using 26015-1-AP

    WB analysis using 26015-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26015-1-AP (RNF128 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26015-1-AP

    IHC staining of human liver cancer using 26015-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26015-1-AP (RNF128 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26015-1-AP

    IHC staining of human liver cancer using 26015-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26015-1-AP (RNF128 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26015-1-AP is RNF128 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RNF128 fusion protein Ag22834 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 128
Synonyms GRAIL, ring finger protein 128, RNF128
Calculated Molecular Weight 428 aa, 47 kDa
Observed Molecular Weight 40-47 kDa, 66-70 kDa
GenBank Accession NumberBC063404
Gene Symbol RNF128
Gene ID (NCBI) 79589
RRIDAB_2880336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF128 antibody 26015-1-APDownload protocol
IHC protocol for RNF128 antibody 26015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.

Authors - Mengqi Chu
  • KD Validated
View Article