验证数据展示
经过测试的应用
| Positive WB detected in | COS-7 cells, MOLT-4 cells, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
29092-1-AP targets RPL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, monkey |
| 文献引用反应性 | human |
| 免疫原 | RPL5 fusion protein Ag30837 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L5 |
| 别名 | ribosomal protein L5, MSTP030, Large ribosomal subunit protein uL18, DBA6, 60S ribosomal protein L5 |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC109370 |
| 基因名称 | RPL5 |
| Gene ID (NCBI) | 6125 |
| RRID | AB_2881240 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46777 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian ribosome is a macromolecular assembly of 4 RNA species (rRNAs) and approximately 80 different proteins, including RPL5. RPL5 (ribosome protein L15) is rquired for rRNA maturation and formation of the 60S ribosomal subunits, and defects or mutation in RPL5 is the cause of DBA6.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL5 antibody 29092-1-AP | Download protocol |
| IHC protocol for RPL5 antibody 29092-1-AP | Download protocol |
| IF protocol for RPL5 antibody 29092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep mTOR- and LARP1-dependent regulation of TOP mRNA poly(A) tail and ribosome loading | ||
BMC Mol Cell Biol Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway
| ||
Front Oncol Spatially resolved transcriptomics revealed local invasion-related genes in colorectal cancer | ||
iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges |
