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验证数据展示

  • WB analysis using 27706-1-AP

    WB analysis using 27706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27706-1-AP (RREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27706-1-AP

    IHC staining of human colon cancer using 27706-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 27706-1-AP

    IHC staining of human ovary cancer using 27706-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RREB1 Polyclonal antibody

RREB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27706-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RREB 1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells
Positive IHC detected inhuman colon cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27706-1-AP targets RREB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RREB1 fusion protein Ag26780 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras responsive element binding protein 1
Synonyms RREB 1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT
Calculated Molecular Weight 181 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberNM_001003698
Gene Symbol RREB1
Gene ID (NCBI) 6239
RRIDAB_2880948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family.RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. RREB1 may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)

实验方案

Product Specific Protocols
WB protocol for RREB1 antibody 27706-1-APDownload protocol
IHC protocol for RREB1 antibody 27706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols