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S100A7/Psoriasin Polyclonal antibody

S100A7/Psoriasin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26656-1-AP

Print datasheet

Synonyms

Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c

验证数据展示

  • WB analysis of MCF-7 using 26656-1-AP

    WB analysis of MCF-7 using 26656-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-468 using 26656-1-AP

    WB analysis of MDA-MB-468 using 26656-1-AP

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 26656-1-AP

    IHC staining of human oesophagus cancer using 26656-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 26656-1-AP

    IHC staining of human oesophagus cancer using 26656-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-468 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26656-1-AP targets S100A7/Psoriasin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen S100A7/Psoriasin fusion protein Ag24717 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A7
Synonyms Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c
Calculated Molecular Weight 101 aa, 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC034687
Gene Symbol S100A7/Psoriasin
Gene ID (NCBI) 6278
RRIDAB_2880592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.

实验方案

Product Specific Protocols
WB protocol for S100A7/Psoriasin antibody 26656-1-APDownload protocol
IHC protocol for S100A7/Psoriasin antibody 26656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols