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S1PR1/EDG1 Polyclonal antibody

S1PR1/EDG1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 55133-1-AP

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Synonyms

CD363, CHEDG1, D1S3362, ECGF1, EDG 1, EDG1, S1P receptor 1, S1P receptor Edg 1, S1P1, S1PR1, S1PR1/EDG1



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55133-1-AP targets S1PR1/EDG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sphingosine-1-phosphate receptor 1
Synonyms CD363, CHEDG1, D1S3362, ECGF1, EDG 1, EDG1, S1P receptor 1, S1P receptor Edg 1, S1P1, S1PR1, S1PR1/EDG1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberNM_001400
Gene Symbol S1PR1
Gene ID (NCBI) 1901
RRIDAB_10793721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21453
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S1PR1 (sphingosine-1-phosphate receptor 1), also known as S1P1 or EDG1, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P) (PMID: 18787560). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR1 is widely expressed in various cell types, including endothelial cells and lymphocytes (PMID: 14737169). S1P-S1PR1 plays a major role in lymphocyte egress and chemotaxis, cell proliferation and survival, and tumor angiogenesis and metastasis (PMID: 21102457). The antibody is specific to S1PR1.

实验方案

Product Specific Protocols
WB protocol for S1PR1/EDG1 antibody 55133-1-APDownload protocol
IP protocol for S1PR1/EDG1 antibody 55133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Neural Regen Res

Fingolimod protects against neurovascular unit injury in a rat model of focal cerebral ischemia/reperfusion injury

Authors - Xiao-Yu Zhu
mouseWB

iScience

Global sphingosine-1-phosphate receptor 2 deficiency attenuates neuroinflammation and ischemic-reperfusion injury after neonatal stroke

Authors - Yumi Fukuzaki
ratWB

Int Immunopharmacol

Dexmedetomidine alleviates oxidative stress and mitochondrial dysfunction in diabetic peripheral neuropathy via the microRNA-34a/SIRT2/S1PR1 axis

Authors - Ying Lin
humanIHC, WB, IP

Front Cell Dev Biol

The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.

Authors - Minjeong Yeon
ratWB

Front Pharmacol

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Authors - Han-Qing Pang
human,ratWB,IHC

Phytomedicine

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Authors - Yan Wang