- Featured Product
- KD/KO Validated
SALL4 Polyclonal antibody
SALL4 Polyclonal Antibody for WB,IP,IHC,IF/ICC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB,IP,IHC,IF/ICC,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human testis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Caco-2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24500-1-AP targets SALL4 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | WB,IP,IHC,IF/ICC,ELISA |
| Cited Applications | WB,IHC,IF |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human |
| Immunogen | SALL4 fusion protein Ag17480 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sal-like 4 (Drosophila) |
| Synonyms | DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797 |
| Calculated Molecular Weight | 1053 aa, 112 kDa |
| Observed Molecular Weight | 66-75 kDa |
| GenBank Accession Number | BC111714 |
| Gene Symbol | SALL4 |
| Gene ID (NCBI) | 57167 |
| RRID | AB_2879576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJQ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SALL4, also named Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4 in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SALL4 antibody 24500-1-AP | Download protocol |
| IHC protocol for SALL4 antibody 24500-1-AP | Download protocol |
| IF protocol for SALL4 antibody 24500-1-AP | Download protocol |
| IP protocol for SALL4 antibody 24500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin. | ||
Cell Signal miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis. | ||
Sci Rep MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1. | ||
Pathol Int Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1 | ||
J Mol Histol Expression and clinical significance of SALL4 and β-catenin in colorectal cancer. |
