SIRT1 Antibody 60303-1-Ig

Tested Applications

Applications:

ELISA, WB, IP, IHC, IF
Application not tested? Please see our Guarantee

WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HeLa cells.

Western blot analysis of SIRT1 in various cell lines using Proteintech antibody 60303-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours

IP Result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug.

Immunohistochemistry of paraffin-embedded human testis tissue slide using 60303-1-Ig( SIRT1 Antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 60303-1-Ig( SIRT1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:

human, mouse

Positive WB detected in:

HEK-293 cells, HeLa cells, K-562 cells

Positive IP detected in:

HeLa cells

Positive IHC detected in:

human testis tissue

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:1000-1:10000

IP : 0.5-4.0 ug for IP and 1:500-1:5000 for WB

IHC : 1:20-1:200

IF : 1:50-1:500

Product Information

Source:

Mouse

Purification method:

Protein A purification

Isotype:

IgG2b

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

SIRT1 fusion protein ag17677

Full name:

sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)

Calculated molecular weight:

747aa,82 kDa

Observed molecular weight:

110-130 kDa

GenBank accession number:

BC012499

Gene ID (NCBI):

23411

Gene symbol

SIRT1

Synonyms

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

Background

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. It regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signalling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, insulin receptor signalling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Showing 1- [0] of [0] Publications

WB Figures

WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HeLa cells.

Western blot analysis of SIRT1 in various cell lines using Proteintech antibody 60303-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 60303-1-Ig( SIRT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 60303-1-Ig( SIRT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human testis tissue slide using 60303-1-Ig( SIRT1 Antibody) at dilution of 1:50 (under 10x lens)