验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22524-1-AP targets DAT in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | DAT fusion protein Ag18297 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 |
| 别名 | SLC6A3, DAT1, DA transporter |
| 计算分子量 | 620 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC133003 |
| 基因名称 | DAT |
| Gene ID (NCBI) | 6531 |
| RRID | AB_2879116 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01959 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DAT antibody 22524-1-AP | Download protocol |
| IHC protocol for DAT antibody 22524-1-AP | Download protocol |
| IF protocol for DAT antibody 22524-1-AP | Download protocol |
| IP protocol for DAT antibody 22524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits | ||
Mov Disord Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration | ||
Phytomedicine Gastrodin relieves Parkinson's disease-related motor deficits by facilitating the MEK-dependent VMAT2 to maintain dopamine homeostasis | ||
J Affect Disord Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats | ||
Br J Pharmacol Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission. |
