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  • KD/KO Validated

SMAD3 Polyclonal antibody

SMAD3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25494-1-AP

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Synonyms

hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3



经过测试的应用

Positive WB detected inJurkat cells, A431 cells, A549 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25494-1-AP targets SMAD3 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, canine, Bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMAD family member 3
Synonyms hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_005902
Gene Symbol SMAD3
Gene ID (NCBI) 4088
RRIDAB_2880102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP84022
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

实验方案

Product Specific Protocols
WB protocol for SMAD3 antibody 25494-1-APDownload protocol
IHC protocol for SMAD3 antibody 25494-1-APDownload protocol
IF protocol for SMAD3 antibody 25494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Circulation

Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation.

Authors - Yoko Kojima
humanWB

Cell Death Differ

REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.

Authors - Chan Jiao
humanWB

Oncogene

The β-catenin/TCF-4-LINC01278-miR-1258-Smad2/3 axis promotes hepatocellular carcinoma metastasis.

Authors - Wei-Juan Huang
  • KD Validated
humanWB,IHC,IF

Stem Cell Reports

Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.

Authors - Wen-Li Deng
  • KD Validated
ratWB

Oxid Med Cell Longev

WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.

Authors - Xiaoyu Zhou
ratWB

Int J Nanomedicine

A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway.

Authors - Ba Hieu Tran