验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12513-1-AP targets SMARCAL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | SMARCAL1 fusion protein Ag3194 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 |
| 别名 | Sucrose nonfermenting protein 2-like 1, hHARP, HepA-related protein, HepA related protein, HARP |
| 计算分子量 | 954 aa, 106 kDa |
| 观测分子量 | 106 kDa |
| GenBank蛋白编号 | BC016482 |
| 基因名称 | SMARCAL1 |
| Gene ID (NCBI) | 50485 |
| RRID | AB_2191690 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZC9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCAL1 is identified as a factor that favors tumor immune evasion by a dual mechanism involving both the suppression of innate immune signaling and the induction of PD-L1-mediated immune checkpoint responses. Mechanistically, SMARCAL1 limits endogenous DNA damage, thereby suppressing cGAS-STING-dependent signaling during cancer cell growth. Simultaneously, it cooperates with the AP-1 family member JUN to maintain chromatin accessibility at a PD-L1 transcriptional regulatory element, thereby promoting PD-L1 expression in cancer cells (PMID: 38301646).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMARCAL1 antibody 12513-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Total Environ LncRNA MT1DP promotes cadmium-induced DNA replication stress by inhibiting chromatin recruitment of SMARCAL1. | ||
J Exp Clin Cancer Res RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma
| ||
Cancer Lett Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer |
