验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, human heart tissue, Raji cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
14015-1-AP targets STAG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | STAG1 fusion protein Ag5123 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | stromal antigen 1 |
| Synonyms | His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1 |
| Calculated Molecular Weight | 144 kDa |
| Observed Molecular Weight | 155 kDa |
| GenBank Accession Number | BC064699 |
| Gene Symbol | STAG1 |
| Gene ID (NCBI) | 10274 |
| RRID | AB_2194759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WVM7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STAG1 antibody 14015-1-AP | Download protocol |
| IHC protocol for STAG1 antibody 14015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1. |
