- Featured Product
- KD/KO Validated
CD71 Polyclonal antibody
CD71 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10084-2-AP targets CD71 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB,IHC,IF,Cell treatment |
| Tested Reactivity | human |
| Cited Reactivity | human, chicken, monkey, pig, duck |
| Immunogen | CD71 fusion protein Ag0123 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transferrin receptor (p90, CD71) |
| Synonyms | CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC001188 |
| Gene Symbol | TFRC |
| Gene ID (NCBI) | 7037 |
| ENSEMBL Gene ID | ENSG00000072274 |
| RRID | AB_2240403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02786 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD71 antibody 10084-2-AP | Download protocol |
| IHC protocol for CD71 antibody 10084-2-AP | Download protocol |
| IF protocol for CD71 antibody 10084-2-AP | Download protocol |
| IP protocol for CD71 antibody 10084-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Acta Neuropathol Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy. | ||
Cell Death Differ Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis | ||
Acta Biomater Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes | ||
Cancer Res Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models. | ||
